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A comparative ultrastructural study of pit membranes with plasmodesmata associated thickenings in four angiosperm species Naturalis
Rabaey, D.; Lens, F.; Huysmans, S.; Smets, E.; Jansen, S..
Recent micromorphological observations of angiosperm pit membranes have extended the number and range of taxa with pseudo-tori in tracheary elements. This study investigates at ultrastructural level (TEM) the development of pseudo-tori in the unrelated Malus yunnanensis, Ligustrum vulgare, Pittosporum tenuifolium, and Vaccinium myrtillus in order to determine whether these plasmodesmata associated thickenings have a similar developmental pattern across flowering plants. At early ontogenetic stages, the formation of a primary thickening was observed, resulting from swelling of the pit membrane in fibre-tracheids and vesselelements. Since plasmodesmata appear to be frequently, but not always, associated with these primary pit membrane thickenings, it remains...
Tipo: Article / Letter to the editor Palavras-chave: Ontogeny; Pseudo-torus; Tracheary element; Pit membrane; Bordered pit; Wood ultrastructure; 42.56.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/430078
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A comparison of paraffin and resin-based techniques used in bark anatomy Naturalis
Hamann, T.; Smets, E.; Lens, F..
Bark anatomy is an unappreciated discipline in plant systematics, despite its great potential to reveal systematically informative features. In this paper, main reasons for the lack of detailed bark anatomical data in many plant families are identified, including problems with sectioning, terminological issues, and difficulties in observation of dilated stems. We deal with these problems by focusing on two aspects: (1) compare, discuss and improve existing sectioning and maceration techniques using two species with soft and hard bark tissues; and (2) discuss the best way to collect stem bark samples. We hope that this paper will stimulate inclusion of bark anatomical data in future systematic studies.
Tipo: Article / Letter to the editor Palavras-chave: Bark anatomy; Light microscopy; Macerations; Plant systematics; Phloem; Sectioning; 42.54.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/421834
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A search for phylogenetically informative wood characters within Lecythidaceae s.l. Naturalis
Lens, F.; Baas, P.; Jansen, S.; Smets, E..
The wood structure of 71 species representing 24 genera of the pantropical Lecythidaceae s.l., including the edible Brazil nuts (Bertholletia excelsa) and the spectacular cannon-ball tree (Couroupita guianensis), was investigated using light and scanning electron microscopy. This study focused on finding phylogenetically informative characters to help elucidate any obscure evolutionary patterns within the family. The earliest diverging subfamily Napoleonaeoideae has mixed simple/scalariform vessel perforations, scalariform vessel-ray pitting, and high multiseriate rays, all features that are also present in Scytopetaloideae. The wood structure of Napoleonaea is distinct, but its supposed close relative Crateranthus strongly resembles Scytopetaloideae. The...
Tipo: Article / Letter to the editor Palavras-chave: Ericales; Lecythidaceae s.l.; Lecythidaceae s.s.; Napoleonaeaceae; Scytopetalaceae; Systematic wood anatomy; 42.48.
Ano: 2007 URL: http://www.repository.naturalis.nl/record/422216
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An extension of the Plant Ontology project supporting wood anatomy and development research Naturalis
Lens, F.; Cooper, L.; Gandolfo, M.A.; Groover, A.; Jaiswal, P.; Lachenbruch, B.; Spicer, R.; Staton, M.E.; Stevenson, D.W.; Walls, R.L.; Wegrzyn, J..
The Wood Ontology project will provide a structured vocabulary and database resource that will be valuable for all scientists, including the IAWA community. To maximizethe utility of the resource and analyses it empowers, it is important for researchers to adopt the use of the ontology terms in the collection and dissemination of their data. The PO website (www.plantontology.org) is the main portal for the Plant Ontology, and presents the current version.
Tipo: Article / Letter to the editor Palavras-chave: Plant Ontology; Wood anatomy and development; Plant morphology; Structured vocabulary; Database resource; 42.40.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/429515
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Comparative wood anatomy of Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Vaccinioideae, Ericaceae s.l.) Naturalis
Lens, F.; Smets, E.; Jansen, S..
The wood anatomical structure of 11 out of 13 genera from four tribes of the Vaccinioideae, namely Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Ericaceae s.l.), is described using light and scanning electron microscopy. Several features of the secondary xylem support the tribal classification based on molecular data: arrangement of vessel-ray pitting, height of multiseriate rays and the shape of the body ray cells. Oxydendreae are clearly defined from the other representatives by various wood anatomical features. Gaultherieae can be distinguished from Lyonieae by differences in vessel perforation plates, vessel-ray pitting, height and structure of multiseriate rays, and occurrence of prismatic crystals, but the wood of Andromedeae s.s. is...
Tipo: Article / Letter to the editor Palavras-chave: Comparative wood anatomy; Andromedeae; Gaultherieae; Lyonieae; Oxydendreae; Vaccinioideae; Ericaceae; Andromedeae sensu Stevens; Pith structure; 42.58.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/424620
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Comparative wood anatomy of epacrids (Styphelioideae, Ericaceae s.l.) Naturalis
Lens, F.; Gasson, P.; Smets, E.; Jansen, S..
The wood anatomy of 16 of the 37 genera within the epacrids (Styphelioideae, Ericaceae s.l.) is investigated by light and scanning electron microscopy. Several features in the secondary xylem occur consistently at the tribal level: arrangement of vessel‐ray pits, distribution of axial parenchyma, ray width, and the presence and location of crystals. The primitive nature of Prionoteae and Archerieae is supported by the presence of scalariform perforation plates with many bars and scalariform to opposite vessel pitting. The wood structure of Oligarrheneae is similar to that of Styphelieae, but the very narrow vessel elements, exclusively uniseriate rays and the lack of prismatic crystals in Oligarrheneae distinguish these two tribes. The secondary xylem of...
Tipo: Article / Letter to the editor Palavras-chave: Ecological wood anatomy; Epacrids; Ericaceae s.l.; Styphelioideae; Systematic wood anatomy; 42.56.
Ano: 2003 URL: http://www.repository.naturalis.nl/record/431740
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Comparative wood anatomy of the blueberry tribe (Vaccinieae, Ericaceae s.l.) Naturalis
Lens, F.; Kron, K.A.; Luteyn, J.L.; Smets, E.; Jansen, S..
Wood samples of 111 Vaccinieae specimens (Vaccinioideae, Ericaceae s.l.) representing 98 species and 26 genera are investigated with light microscopy and scanning electron microscopy. The wood structure of Vaccinieae delivers taxonomically important characters that can be used to define some subclades within the tribe. The wood of the large polyphyletic genus Vaccinium strongly resembles non-vaccinioid members of the family, which are characterized by bordered vessel-ray pits and relatively narrow (2- to 4-seriate) and low multiseriate rays (often less than 1000 mm) with exclusively or mainly procumbent body ray cells. The East Malesian clade, Meso-American/Caribbean clade, and the Andean clade show a combination of wood anatomical features that is lacking...
Tipo: Article / Letter to the editor Palavras-chave: Blueberries; Comparative wood anatomy; Ericaceae; Neotropics; Secretory ducts; Systematics; Vaccinieae; 42.56.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/423810
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Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.) Naturalis
Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407307
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Contributions to the Wood Anatomy of the Rubioideae (Rubiaceae) Naturalis
Jansen, S.; Lens, F.; Ntore, S.; Piesschaert, F.; Robbrechf, E.; Smets, E..
The secondary xylem of Craterispermeae, Coussareeae, Morindeae s.str., Prismatomerideae, Pauridiantheae, Urophylleae, and Triainolepideae (Rubiaceae, Rubioideae) is described and illustrated in detail. Genera that were previously placed in the Morindeae or Psychotrieae such as Damnacanthus, Lasianthus, Saldinia, and Trichosfachys are also included. Wood anatomical characters are compared with recent phylogenetic insights into the study group on the basis of molecular data. The observations demonstrate that the delimitation and separation of several taxa from the former Coussareeae/Morindeae/Prismatomerideae/Psychotrieae aggregate is supported by wood anatomical data. The Coussareeae can be distinguished from the other Rubioideae by their scanty parenchyma,...
Tipo: Article / Letter to the editor Palavras-chave: Rubiaceae; Rubioideae; Systematic wood anatomy; 42.58.
Ano: 2001 URL: http://www.repository.naturalis.nl/record/421859
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Do quantitative vessel and pit characters account for ion-mediated changes in the hydraulic conductance of angiosperm xylem? Naturalis
Jansen, S.; Gortan, E.; Lens, F.; Assunta Lo Gullo, M.; Salleo, S.; Scholtz, A.; Stein, A.; Trifilò, P.; Nardini, A..
• The hydraulic conductance of angiosperm xylem has been suggested to vary with changes in sap solute concentrations because of intervessel pit properties. • The magnitude of the ‘ionic effect’ was linked with vessel and pit dimensions in 20 angiosperm species covering 13 families including six Lauraceae species. • A positive correlation was found between ionic effect and vessel grouping parameters, especially the portion of vessel walls in contact with neighbouring vessels. Species with intervessel contact fraction (FC) values < 0.1 showed an ionic effect between 2% and 17%, while species with FC values > 0.1 exhibited a response between 10% and 32%. The ionic effect increased linearly with the mean fraction of the total vessel wall area occupied...
Tipo: Article / Letter to the editor Palavras-chave: Angiosperms; Hydraulic conductivity; Intervessel pit membrane; Ionic effect; Vessel grouping; Wood anatomy; 42.41.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/423644
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Ecological trends in the wood anatomy of Vaccinioideae (Ericaceae s.l.) Naturalis
Lens, F.; Luteyn, J.L.; Smets, E.; Jansen, S..
The ecological wood anatomy of 128 vaccinioid wood samples (including 115 species, 35 genera), collected between 39°S and 60°N latitude and 10 m to 3400 m altitude is studied. Several wood anatomical features within the subfamily, viz. tangential vessel diameter, average length of tracheary elements, height of multiseriate rays, and presence of prismatic crystals are negatively correlated with increasing latitude, while vessel density and helical thickenings show a positive correlation with increasing latitude. Similar latitudinal trends are found within the genus Vaccinium (31 species studied). The correlation between various wood anatomical features and latitude is surprisingly high despite the fact that most tropical species grow in montane regions,...
Tipo: Article / Letter to the editor Palavras-chave: Altitude; Ecological and functional wood anatomy; Ericaceae; Latitude; Vaccinioideae; Vaccinium; 42.58.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/424624
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Embolism resistance as a key mechanism to understand adaptive plant strategies Naturalis
Lens, F.; Tixier, A.; Cochard, H.; Sperry, J.S.; Jansen, S.; Herbette, S..
One adaptation of plants to cope with drought or frost stress is to develop wood that is able to withstand the formation and distribution of air bubbles (emboli) in its water conducting xylem cells under negative pressure. The ultrastructure of interconduit pits strongly affects drought-induced embolism resistance, but also mechanical properties of the xylem are involved. The first experimental evidence for a lower embolism resistance in stems of herbaceous plants compared to stems of their secondarily woody descendants further supports this mechanical-functional trade-off. An integrative approach combining (ultra)structural observations of the xylem, safetyefficiency aspects of the hydraulic pipeline, and xylem–phloem interactions will shed more light on...
Tipo: Article / Letter to the editor Palavras-chave: Embolism resistance; Adaptive plant strategies; Drought stress; Air bubbles (emboli); Xylem; 42.42.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/453852
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Evolution of fruit and seed characters in the Diervilla and Lonicera clades (Caprifoliaceae, Dipsacales) Naturalis
Jacobs, B.; Lens, F.; Smets, E..
Background and Aims: The Diervilla and Lonicera clades are members of the family Caprifoliaceae (Dipsacales sensu Donoghue et al., 2001, Harvard Papers in Botany 6: 459–479). So far, the intergeneric relationships of the Lonicera clade and the systematic position of Heptacodium remain equivocal. By studying fruit and seed morphology and anatomy, an attempt is made to clarify these issues. In addition, this study deals with the evolution of fruit and seed characters of the Diervilla and Lonicera clades with reference to allied taxa. Methods: Light and scanning electron microscopy were used for the morphological and anatomical investigations. Phylogenetic analyses were carried out by applying the parsimony and Bayesian inference optimality criteria....
Tipo: Article / Letter to the editor Palavras-chave: Diervilla; Weigela; Symphoricarpos; Lonicera; Triosteum; Leycesteria; Heptacodium; Caprifoliaceae; Dipsacales; Fruit; Seed; Evolution; 42.56.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/421854
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Flowering-time genes modulate meristem determinacy and growth form in Arabidopsis thaliana Naturalis
Melzer, S.; Lens, F.; Gennen, J.; Vanneste, S.; Rohde , A.; Beeckman, T..
Plants have evolved annual and perennial life forms as alternative strategies to adapt reproduction and survival to environmental constraints. In isolated situations, such as islands, woody perennials have evolved repeatedly from annual ancestors1. Although the molecular basis of the rapid evolution of insular woodiness is unknown, the molecular difference between perennials and annuals might be rather small, and a change between these life strategies might not require major genetic innovations2,3. Developmental regulators can strongly affect evolutionary variation4 and genes involved in meristem transitions are good candidates for a switch in growth habit. We found that the MADS box proteins SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FRUITFULL...
Tipo: Article / Letter to the editor Palavras-chave: Flowering-time; Meristem determinacy; Growth form; Arabidopsis thaliana; Insular woodiness; Secondary growth; Annual life forms; 42.56.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/429531
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Global convergence in the vulnerability of forests to drought Naturalis
Choat, B.; Jansen, S.; Brodribb, T.J.; Cochard, H.; Delzon, S.; Bhaskar, R.; Bucci, S.J.; Feild, T.S.; Gleason, S.M.; Hacke, U.G.; Jacobsen, A.L.; Lens, F.; Maherali, H.; Martínez-Vilalta, J.; Mayr, S.; Mencuccini, M.; Mitchell, P.J.; Nardini, A.; Pittermann, J.; Pratt, R.B.; Sperry, J.S.; Westoby, M.; Wright, I.J.; Zanne, A.E..
Shifts in rainfall patterns and increasing temperatures associated with climate change are likely to cause widespread forest decline in regions where droughts are predicted to increase in duration and severity. One primary cause of productivity loss and plant mortality during drought is hydraulic failure. Drought stress creates trapped gas emboli in the water transport system, which reduces the ability of plants to supply water to leaves for photosynthetic gas exchange and can ultimately result in desiccation and mortality. At present we lack a clear picture of how thresholds to hydraulic failure vary across a broad range of species and environments, despite many individual experiments. Here we draw together published and unpublished data on the...
Tipo: Article / Letter to the editor Palavras-chave: Global convergence vulnerability forests drought climate change drought-induced forest decline 42.44; 43.47.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/453830
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Insular woodiness on the Canary Islands: a remarkable case of convergent evolution Naturalis
Lens, F.; Davin, N.; Smets, E.; Arco, M. del.
Premise of research. One of the most conspicuous aspects of island floras is the relatively high proportion of woody species. Often, but not always, these woody species have developed wood on the islands and have evolved from herbaceous continental ancestors, a phenomenon known as insular woodiness. Shifts from herbaceousness toward increased woodiness also occur on continents (the broader term “secondary woodiness” is more appropriate here and includes insular woodiness), but comprehensive worldwide knowledge about secondary woodiness within angiosperms remains lacking. We update hypotheses regarding the herbaceous ancestry of woody Canarian lineages in a molecular phylogenetic context and investigate the possible link of secondary woodiness and...
Tipo: Article / Letter to the editor Palavras-chave: Canary Islands convergent evolution insular woodiness molecular phylogeny protracted juvenilism seconday woodinessq wood anatomy 42.21; 42.44.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/453855
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Intervascular pit membranes with a torus in the wood of Ulmus (Ulmaceae) and related genera Naturalis
Jansen, S.; Choat, B.; Vinckier, S.; Lens, F.; Schols, P.; Smets, E..
• The distribution of intervascular pit membranes with a torus was investigated in juvenile wood samples of 19 species of Ulmus and seven related genera. • A staining solution of safranin and alcian blue (35 : 65) was recommended to distinguish torus-bearing pit membranes using light microscopy. • Intervascular pit membranes connecting relatively wide vessel elements resembled those of most angiosperms, as they were of uniform thickness. By contrast, bordered pit pairs with round to oval pit apertures and indistinct pit canals that connected narrow (incomplete) vessel elements or vascular tracheids with distinct helical thickenings were frequently characterized by a torus in ring-porous wood samples of Ulmus and Zelkova. Tori were lacking in...
Tipo: Article / Letter to the editor Palavras-chave: Ulmus (elm); Ulmaceae; Pit membrane; Torus; Pit structure; Wood anatomy; Tracheary elements; 42.58.
Ano: 2004 URL: http://www.repository.naturalis.nl/record/423388
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Micromorphology and systematic distribution of pit membrane thickenings in Oleaceae: Tori and pseudo-tori Naturalis
Rabaey, D.; Huysmans, S.; Lens, F.; Smets, E.; Jansen, S..
Recent studies on the functional significance of pit membranes in water conducting cells have renewed general interest in their micromorphology. At least two types of pit membrane thickenings have been described in angiosperm families, i.e. genuine tori and pseudo-tori. This study explores the distribution and morphology of pit membrane thickenings in 69 species and 23 genera within Oleaceae using light and electron microscopy. Torus-bearing pit membranes are confirmed for Osmanthus, and new records are reported for Chionanthus retusa, Picconia azorica, and P. excelsa, but not for the other species studied of Chionanthus. This infrageneric variation suggests that tori represent a plastic feature that has evolved more than once within the family as the...
Tipo: Article / Letter to the editor Palavras-chave: Electron microscopy; Pit aperture; Pit membrane; Plasmodesmata; Pseudo-torus; Torus; Tracheid; Vessel element; 42.56.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/429536
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Palynological Characters and Their Phylogenetic Signal in Rubiaceae Naturalis
Dessein, S.; Ochoterena, H.; De Block, P.; Lens, F.; Robbrecht, E.; Schols, P.; Smets, E.; Vinckier, S.; Huysmans, S..
In the 1990s Rubiaceae became a hot spot for systematists, mainly due to the comprehensive treatment of the family by Robbrecht in 1988. Next to the exploration of macromolecular characters to infer the phylogeny, the palynology of Rubiaceae finally received the attention it deserves. This article aims to present a state-of-the-art analysis of the systematic palynology of the family. The range of variation in pollen morphology is wide, and some of the pollen features are not known from other angiosperm taxa; e.g., a looplike or spiral pattern for the position of apertures in pantoaperturate grains. We compiled an online database at the generic level for the major pollen characters and orbicule presence in Rubiaceae. An overview of the variation is...
Tipo: Article / Letter to the editor Palavras-chave: Rubiaceae; Pollen morphology; Palynology; Phylogeny.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407298
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Palynological Variation in Balsaminoid Ericales. I. Marcgraviaceae Naturalis
Lens, F.; Dressler, S.; Vinckier, S.; Janssens, S.; Dessein, S.; Van Evelghem, L.; Smets, E..
Background and Aims Marcgraviaceae are a rather small family of seven genera and approx. 130 neotropical species. This study aims to present a detailed palynological survey of the family in order to comment on the intrafamily relationships and possible correlations with pollinators. Methods In total, 119 specimens representing 67 species and all genera are observed using light microscopy and scanning electron microscopy. Furthermore, eight species from five genera are studied with transmission electron microscopy. Key Results Our results show that pollen grains of Marcgraviaceae are small (20–35 mm), have three equatorial apertures, granules on the colpus membrane, oblate spheroidal to prolate spheroidal shapes, mainly psilate to perforate ornamentations,...
Tipo: Article / Letter to the editor Palavras-chave: Balsaminoids; Ericales; Marcgraviaceae; Neotropics; Orbicules; Palynology; SEM; TEM.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407283
Registros recuperados: 41
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